Genome-assisted biogeography of Phaeocystales – Zoltan Füssy
Genome-assisted biogeography of Phaeocystales, based on genome sequence data from cultured strains and metagenome-assembled genomes. The data are binned by taxonomic assignment to tentative lineages, as determined from a multi-gene phylogeny, and by sample size fraction. An overview of the life cycle of colony-forming Phaeocystis is provided in the upper right panel – abundance in large size fractions suggests the development of the colonial stage or symbiotic relationships, such as those known to form with Acanthareans.
Water collection with a trace metal clean rosette – Rob Lampe
JCVI and Scripps scientists aboard R/V Atlantis in the Pacific Ocean off the coast of Big Sur, California, recovering the ship’s main trace metal clean rosette to gather measurements (e.g., temperature, salinity, nutrients) during the cruise.
Reference: Lampe et al_Nature Communications_2023
Deployment of DeepISCA – Estelle Clerc
The DeepISCA is attached to an independent deployment wire, before being lowered to the desired depth. Here in our deployments in Mallorca, in October 2023, we sampled chemotactic bacteria at 200 m in the ocean. The DeepISCA allows for depth-specific deployment of the ISCA below the surface, while avoiding turbulence and contamination of the wells by unwanted bacteria.
DeepISCA – Estelle Clerc
Two copies of DeepISCAs enable us to deploy 16 ISCAs at once and characterize the chemotactic response of marine bacteria to 18 different substances. In Mallorca in October 2023, we deployed a suite of chemicals ranging from labile to refractory carbon compounds, to characterize the response of marine communities along the depth axis.
ISCA deployment – Estelle Clerc
ISCAs are deployed in small deployment boxes in surface water.
In situ chemotaxis assay (ISCA) – Estelle Clerc
The ISCA is credit card-sized with 20 wells distributed in a 5 x 4 well array, each linked to the external aquatic environment by a small port. Chemoattractants loaded into the wells diffuse in the environment via the port, and chemotactic microbes respond by swimming through the port into the well.
Source: Clerc et al_Journal of Visualized Experiments_2020